ANTIOXIDANT AND ANTICANCER ACTIVITIES OF PROTEIN HYDROLYSATES FROM SEEDS OF HAIRY BASIL Ocimum basilicum L.

Abstract

Nowadays, Thailand is going to be aging society. Consuming healthy food and dietary supplement are interesting and becoming increasing by consumer. Free radicals are atoms or compounds which have unpair electron at the high-energy outest ring of orbital. Therefore, they quickly react with other molecules within cells in human body. Normally, there are mechanisms to get rid of them out of human cells but there are also partial free radicals that cannot be eliminated. These uneliminated free radicals causes cell damages which lead to many diseases such as coronary thrombosis, cancer, Alzheimer’s and arthritis. Thus this research aimed to find an optimized condition in production of antioxidant protein hydrolysates from hairy basil seeds by digestion of 3 enzymes, proteaseG6, pepsin-pancreatin, and papain, and evaluate ability of free radical scavenging of obtained proteins from each enzyme digestion. After ultrafiltration the protein hydrolysates were detained. The antioxidant activities were analyzed by 4 techniques, DPPH, ABTS, NO and H2O2. From results, digestion with 5.8 x 105 Du proteaseG6 for 270 min produced highest effective protein hydrolysates from hairy basil seeds. Besides, DPPH and ABTS analysis revealed that protein hydrolysates from hairy basil seeds could decrease free radical by electron transfer and chain breaking. In addition, The study in 5 cancer cell lines, human breast carcinoma cell line (BT4740), human liver carcinoma cell line (HEP-G2), human lung bronchus carcinoma cell line (CHAGO), human colon carcinoma cell line (SW620) and human stomach carcinoma cell line (KATO-III), showed that obtained hydrolysis proteins could inhibit cell proliferation better than doxorubicin which is commercial medicine used in the present.