การค้นหายีนที่ควบคุมโดยโปรตีนคัลมอดุลินในการตอบสนองต่อสภาวะความเครียดจากสิ่งแวดล้อมของข้าว Oryza sativa L. : รายงานวิจัย

Abstract

Environmental stresses such as salinity, drought, heat, or pathogen greatly affect agricultural productivity. Researchers have long been attempting to improve plant tolerance to these adverse conditions. To be successful, knowledge of the mechanisms involved in environmental stress responses of plants in the molecular level is needed. To tolerate stress in plants, Ca2+ signals have been implicated in transducing the initial signals from various environmental changes to elicit appropriate responses. Here, we aimed to identify genes that are regulated by OsCaM1, which is a primary Ca2+ sensor calmodulin from Oryza sativa L. 'KDML 105'. First, five candidate genes that co-expressed with OsCam1-1 (LOC_Os03g20370) were selected. Their expression levels were examined in the three transgenic rice lines overexpressing OsCam1-1 by real-time RT-PCR. Compared with the wild-type and the transgenic control rice line, which was transformed with the vector alone, expression of two genes (LOC_ Os03g16690 and LOC_Os 11 g38800) was slightly altered in the overexpressing lines. We further used cDNAAmplified Fragment Length Polymorphism (cDNA-AFLP) to generate transcriptomic profiles of the Wild-type rice and the transgenic rice overexpressing OsCom 1-1 and identified differentially expressed genes. We found 100 transcript-derived fragments (TDFs) differentially expressed in the transgenic rice compared to that in the wild type. So far, 10 up-regulated genes and 24 downregulated genes were identified from these TDFs. The identification of genes corresponding to these TDFs will enable us to characterize the mechanisms of OsCam1-1 in vivo and how these modulated genes might involve in the environmental stress response.