Hypocholesterolemic property of lactobacillus Thai isolates

Abstract

High level of cholesterol causes various kinds of disease including cardiovascular disease (CVD) which is the most major cause morbidity and mortality worldwide. Control of fat diet, exercise and medication are common methodto reduce cholesterol. It has been reported that Lactobacillusprobiotics can prevent and/or treat hypercholesterolemia in humans. This study aims to search for Lactobacillus Thai isolates with hypocholesterolemicproperty. Fifty –one Lactobacillus isolates from infant and adult feces were tested for bile salt hydrolase (BSH) production and cholesterol lowering activities by colorimetric assay. Nineteen isolates produced BSH and six isolates could assimilate cholesterol in vitro. Out of 19 BSH- producing Lactobacillus, 9 deconjugated sodium taurocholate (TCA) and 10 deconjugated both TCA and sodium glycocholate (GCA). Potential hypocholesterolemicLactobacillus isolates were tested for bile and acid tolerance, 2 isolates (L. fementum Lac31 and L. plantarum L61-1) were found to survive in 0.3%oxgall and acidic pH 3 condition. L. fermentumLac31was tested for hypocholeterolemic effect in BALB/c mice but did not show the effectL. fermentum (Lac31) did not translocate to blood, liver and spleen.